The MDA-MB-231 cell line was established from the pleural effusion of a 51-year-old Caucasian female patient with breast cancer. These cells express epidermal growth factor (EGF) receptors, TGF-α receptors, and the WNT7B oncogene.

Cell Name: Human breast adenocarcinoma cell line

Cell Synonyms: MDA-MB-231; MDA_MB_231; MDA-MB 231; MDA.MB.231; MDA MB 231; MDA MB231; MDA Mb231; MDA-MB231; MDAMB-231; MDAMB231; MDA-231; MDA231; MDA231-BRE; MB231; MD Anderson-Metastatic Breast-231

Catalogue No.: TCH-C248

Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: L-15 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G248

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: Air , Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection

Note:

1. This cell line is recommended to be cultured in Leibovitz's L-15 medium. Leibovitz's L-15 medium should not be exposed to carbon dioxide, as CO₂ will cause cytotoxicity.

2. If you do not have a CO₂-free incubator, you may use DMEM instead of Leibovitz's L-15. When using DMEM medium, a normal 5% CO₂ environment can be applied.

3. The matching dedicated medium is configured for Leibovitz's L-15 by default. If you require the DMEM formulation, please contact the sales team to place your order and note that the medium should be changed to DMEM-H for human breast cancer cells (TCH-C453).