The growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF-α).

The secretion of IGFBPs can be modulated through anti-estrogen treatment.

Cell Name: Human breast adenocarcinoma cell line (STR profile confirmed)

Cell Synonyms: MCF-7; MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7;   ssMCF-7; ssMCF7; MCF7/WT; IBMF-7; MCF7-CTRL

Catalogue No.: TCH-C247

Species: Human
Tissue Origin: Breast
Disease Characteristics: Breast cancer
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: MEM + 10% FBS + 1% Glutamax + 1% NEAA + 1% Sodium Pyruvate + 10 µg/mL Human recombinant insulin + 1% P/S

Matching Culture Medium No.: TCH-G247

Passage Ratio: 1:3–1:6, change medium every 2–3 days

Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma