- Parameters
- Detail
- Cell Info
- References
Cell Name: Human mammary epithelial cell line
Cell Synonyms: MCF-10A; MCF 10A; MCF.10A; MCF10A; MCF10a; MCF-10 Attached; Michigan Cancer Foundation-10A
Catalogue No.: TCH-C246
Species: Human
Tissue Origin: Mammary Gland
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: D/F12 + 5% HS + 20 ng/mL Human EGF + 100 ng/mL Cholera Toxin + 500 ng/mL Hydrocortisone + 1% P/S
Matching Medium Catalog Number: TCH-G246
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection