LS513 is a colorectal cancer cell line isolated in 1985 from a primary tumor biopsy of a 63-year-old Caucasian male patient diagnosed with a mucin-secreting cecal tumor (Dukes' C stage) located at the ileocecal valve.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: LS513; LS-513; LS 513

Catalogue No.: TCH-C241
Species: Human
Tissue Origin: Colorectal
Disease Characteristics: Colorectal cancer

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G241

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection