LS 174T is a trypsinized variant of the LS 180 human colon adenocarcinoma cell line. It is more easily subcultured than its parent and, like LS 180, produces large amounts of carcinoembryonic antigen (CEA). Electron microscopy studies reveal an abundance of microfilaments and cytoplasmic mucin vacuoles.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: LS 174T; Ls174T; LS174t; Ls-174-T; LS-174-T; LS 174 T; LS-174T; Ls-174T; LS174T; LS-174; LS174

Catalogue No.: TCH-C240

Species: Human

Tissue Origin: Rectum

Disease Characteristics: Colorectal Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G240
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection