The LNCaP clone FGC was isolated in 1977 by JS Horoszewicz et al. from a fine needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) diagnosed with metastatic prostate cancer.
Cell Name: Human prostate adenocarcinoma cell line
Cell Synonyms: Lncap; LNCAP; LNCap; Lymph Node Carcinoma of the prostate
Catalogue No.: TCH-C238 Species: Human Tissue Origin: Prostate Disease Characteristic: Prostate Cancer
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days Doubling Time: 72-96 h Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201 Quality Control: Negative for bacteria, fungi, and mycoplasma detection
Precautions for LNCaP Cell Culture:
These cells do not form a uniform monolayer but instead grow in colonies.
The cells acidify the medium rapidly and grow slowly. Therefore, they should not be disturbed within 48 hours after passaging.
Ordinary TC-treated flasks or dishes do not provide sufficient cell adhesion, making cultivation more challenging. It is recommended to use 0.1% gelatin (Hycyte® GUGL-R001) coating.
During transportation, most cells may detach from the flask bottom and float in the medium. Follow the post-receipt handling instructions to restore normal conditions.
If floating cells or clumps are observed upon receipt, proceed with the following steps:
(1) Transfer all medium from the flask into a sterile centrifuge tube and centrifuge to collect the cells (1200 rpm, 3 min).
(2) Discard the supernatant, resuspend the cells in PBS, combine them into one tube, mix gently by shaking the tube, and centrifuge again (1200 rpm, 3 min).
(3) Discard the supernatant, add about 1 mL of 0.25% trypsin, and resuspend the cells by gently shaking the tube (do not pipette). Incubate in a cell culture incubator for approximately 3 minutes to digest the cells.
(4) After digestion, gently pipette the cell suspension to disperse clumps. Add 5 mL of serum-containing medium to stop digestion, then centrifuge (1200 rpm, 3 min).
(5) Discard the supernatant, resuspend the cells in 5–7 mL of complete medium, and gently pipette repeatedly. Seed the cells into a sterile T25 flask (pre-wet the flask with the corresponding medium beforehand).