Established from a poorly differentiated squamous cell carcinoma of the esophagus (tumor invading adjacent structures) resected from the upper (cervical) esophagus of a 49-year-old Japanese female following radiotherapy; described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x), and capable of forming tumors in nude mice.

Cell Name: Human esophageal squamous cell carcinoma cell line

Cell Synonyms: KYSE-150; KYSE 150; KYSE150; Kyse150; KY150

Catalogue No.: TCH-C236

Species: Human

Tissue Origin: Esophagus

Disease Characteristics: Esophageal squamous cell carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G236

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection