KU812 is a basophil cell line isolated from the peripheral blood of a 38-year-old Asian male patient with chronic myeloid leukemia (CML).

Cell Name: Human chronic myelogenous leukemia basophilic cell line

Cell Synonyms: KU812; KU812; KU-812; KU.812; KU 812

Catalogue No.: TCH-C235
Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristic: Chronic Myelogenous Leukemia
Morphology: Myeloblast-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 20% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G235

Passage Ratio: 5×10⁵-2×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 60-80 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection