Granulosa cell tumor. Possible to produce progesterone after HCG addition. Cell growth is slow.
Isolated from human ovarian granulosa cell tumor tissue, the activity of the Aromatase enzyme increases under the stimulation of FSH and hMG.
Cell Name: Human granulosa cell tumor cell line
Cell Synonyms: KGNCatalogue No.: TCH-C230Species: HumanTissue Origin: OvaryDisease Characteristics: Granulosa Cell TumorMorphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: D/F12 + 10% FBS+ 1% P/SMatching Medium Catalog Number: TCH-G230
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 daysDoubling Time: 48-72 hCulture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201Quality Control: Negative for bacteria, fungi, and mycoplasma detection