The human gastric cancer cell line KATO III was derived from a 55-year-old Asian male with metastatic gastric cancer. Source sites: pleural effusion, supraclavicular and axillary lymph nodes, and Douglas cul-de-sac.

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: KATO III
Catalogue No.: TCH-C228
Species: Human
Tissue Origin: Stomach
Disease Characteristic: Gastric Cancer
Morphology: Round
Growth Properties: Semi-adherent / semi-suspension
Culture Medium: IMDM + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G228

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection