Product Name：Luciferase Reporter K-562 Cell Line

HyCyte cell line：HyCyte K-562-Luc

Catalog No.：TCH-C226L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：K-562

Species：Human

Morphology：Lymphoblastoid

Growth properties：Suspension

Culture Medium：Iscove’s Modified Dulbecco Medium(IMDM) supplemented with 10 % FBS, 4.0 ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (TCH-G226L).

Passage：1:3-1:6

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：IMDM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).