This cell line is suitable as a transfection host and was derived from the pleural effusion of a 53-year-old female patient with chronic myelogenous leukemia.

Cell Name: Human chronic myelogenous leukemia cell line (STR profile verified)

Cell Synonyms: K-562; K562; K.562; K 562; KO; GM05372; GM05372E

Catalogue No.: TCH-C226

Species: Human
Tissue Origin: Bone marrow
Disease Characteristics: Chronic myelogenous leukemia
Morphology: Lymphoblast-like
Growth Properties: Suspension growth
Culture Medium: IMDM + 10% FBS + 1% P/S
Recommended Medium Catalog Number: TCH-G226
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 24–48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma