This cell is a clone of the Jurkat-FHCRC cell line (a derivative of the Jurkat cell line). The Jurkat cell line was derived from the peripheral blood of a 14-year-old boy; it can produce large amounts of IL-2 upon induction with either phorbol ester and a lectin or an anti-T3 monoclonal antibody (the production of IL-2 requires both types of inducers); it expresses the T-cell receptor and CD3.

Cell Name: Human T cell leukemia cell line (STR-verified)

Cell Synonyms: Jurkat; JurkatE6-1; Jurkat E6-1; Jurkat Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1

Catalogue No.: TCH-C225

Species: Human
Tissue Origin: Peripheral blood
Disease Characteristics: Acute T lymphocytic leukemia
Morphology: Lymphoblast-like
Growth Characteristics: Suspension growth
Culture System: RPMI-1640 + 10% FBS + 1% GlutaMAX + 1% Sodium Pyruvate + 1% P/S
Matching Medium Number: TCH-G225

Passage Ratio: 1:3-1:6, with medium change every 2-3 days

Doubling Time: 24-48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma