This cell line was derived from a 58-year-old Caucasian male with bladder transitional cell carcinoma. The cells lack desmosomes, contain abundant rough endoplasmic reticulum and prominent microvilli, and express the ras gene.

Cell Name: Human bladder carcinoma cell line (STR authenticated)

Cell Synonyms: J82; J-82; J 82; J82COT; J82 COT

Catalogue No.: TCH-C221

Species: Human
Tissue Origin: Bladder
Disease Feature: Transitional Cell Carcinoma
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture System: MEM + 10% FBS + 1% P/S
Matching Culture Medium Code: TCH-G221
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma