IMR-32 cells were established in April 1967 by W.W. Nichols, J. Lee, and S. Dwight from an abdominal tumor of a 13-month-old male infant. The diagnosis was confirmed as neuroblastoma with partial areas showing organic differentiation. The cell line comprises two cell types: predominantly small neuroblast-like cells and larger clear fibroblastic cells. It has been demonstrated that these cells can be subcultured for over 80 passages.

Cell Name: Human Neuroblastoma Cells
Cell Synonyms: IMR-32
Catalogue No.: TCH-C219
Species: Human
Tissue Origin: —
Disease Characteristics: Neuroblastoma
Morphology: Neuroblast-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G219

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection