This cell can produce IL-2 and TNF-α; it expresses the IL-2 receptor.
Cell Name: Human T cell lymphoma cell line (STR-authenticated)
Cell Synonyms: HuT 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78; HUT78; NCI-H78
Catalogue No.: TCH-C218
Species: Human
Tissue Origin: Skin
Disease Feature: Cutaneous leukemia (Sézary syndrome)
Morphology: Lymphoblast
Growth Characteristics: Suspension growth
Culture System: IMDM + 20% FBS + 1% P/S
Matching Medium No.: TCH-G218
Passage Ratio: 1:3–1:6, change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma