Product Name：Luciferase Reporter HuH-7 Cell Line

HyCyte cell line：HyCyte HuH-7-Luc

Catalog No.：TCH-C217L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：HuH-7

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium： Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,4.0 ug/mL Puromycin, 2mM Glutamax, 1mM Sodium Pyruvate,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G217L）

Passage：1:2-1:3

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).