This cell produces alpha-fetoprotein, pancreatic enzyme α antibody, ceruloplasmin, fibrinogen, fibronectin, and others.

Cell Name: Human hepatocellular carcinoma cell line (STR profile confirmed)
Cell Synonyms: HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39

Catalogue No.: TCH-C217

Species: Human

Tissue Origin: Liver
Disease Characteristic: Hepatocellular carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H +10% FBS + 1% Glutamax + 1% Sodium Pyruvate + 1% P/S
Matching Medium Catalog Number: TCH-G217

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma