This cell line is a hepatoblastoma cell line, which has been reported to produce albumin and alpha-fetoprotein. It is an important cell line derived from liver cancer tissue and is commonly used in biological research to study hepatitis C virus infection, liver cancer, drug metabolism and pharmacokinetics, drug-drug interactions, transporter-mediated efflux inhibition, and the discovery of slowly metabolized drugs.

Cell Name: Human Hepatoblastoma Cells

Cell Synonyms: HuH-6

Catalogue No.: TCH-C216
Species: Human
Tissue Origin: Liver

Disease Characteristics: Hepatoblastoma
Morphology: Epithelial-like

Growth Properties: Adherent growth

Culture Medium: DMEM-H + 10% FBS (Fetal Bovine Serum) + 1% P/S (Penicillin-Streptomycin)
Passage Ratio: 1:2–1:4; change medium every 2–3 days
Doubling Time: 48–60 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; store in liquid nitrogen
Recommended Cryopreservation Solution: HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product Code: GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma detectio