The cell line was originally isolated from a primary tumor in 1964 by Fogh J using transplant culture methods and F12 medium containing 15% FBS. Recently, the established cultured cells have been maintained in serum-supplemented McCoy’s 5a medium. This cell line forms tumors in nude mice and can also induce tumorigenesis in steroid-treated hamsters. The cells synthesize IgA, CEA, TGF-β binding protein, and mucin; express urokinase receptors but show no detectable plasminogen activity; and do not express CD4, though they exhibit surface expression of galactosylceramide (a potential alternative receptor for HIV). The cell line tests positive for the oncogenes c-myc, K-ras, H-ras, N-ras, Myb, sis, and fos; overexpresses the p53 gene, and harbors a G.

Cell Name: Human Colorectal Cancer Cell Line (STR authenticated)

Cell Synonyms:HT-29; HT 29; HT29

Catalogue No.:TCH-C213

Species: Human
Tissue Origin: Colorectum
Disease Characteristic: Colorectal Cancer
Morphology: Epithelial-like
Growth Properties: Adherent
Culture System: McCoy’s 5A + 10% FBS + 1% P/S
Matching Medium No.: TCH-G213

Passage Ratio: 1:3–1:6; change medium every 2–3 days

Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma