The HT-1080 cell line contains an activated N-ras oncogene.

Cell Name: Human fibrosarcoma cell line

Cell Synonyms: HT-1080; Ht-1080; HT 1080; HT1080; HT 1080.T

Catalogue No.: TCH-C212

Species: Human

Tissue Origin: Connective tissue

Disease Characteristic: Fibrosarcoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G212

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection