Product Name：Luciferase/GFP Dual-Reporter HL-60 Cell Line

HyCyte cell line：HyCyte HL-60-Luc-GFP

Catalog No.：TCH-C204LG

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：HL-60

Species：Human

Morphology：Myeloblastoid

Growth properties：Suspension

Culture Medium：Iscove’s Modified Dulbecco Medium(IMDM) supplemented with 20 % FBS, 0.5 ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (TCH-G204LG).

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：IMDM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).