This cell line was established by Collins SJ from the peripheral blood of a 36-year-old Caucasian female with acute promyelocytic leukemia. It can differentiate spontaneously or in response to stimulation by butyrate, hypoxanthine, phorbol myristate acetate (PMA, TPA), DMSO (1% to 1.5%), actinomycin D, and retinoic acid. PMA stimulation induces the secretion of TNF-α. The cells exhibit phagocytic activity and chemotactic responses, are positive for the oncogene myc, and express complement receptors and Fc receptors.

Cell Name: Human promyelocytic leukemia cell line (STR authenticated)

Cell Synonyms: HL-60; HL 60; HL.60; HL60

Catalogue No.: TCH-C204

Species: Human
Tissue Source: Peripheral Blood
Disease Characteristic: Acute granulocytic-monocytic leukemia
Morphology: Myeloblast-like
Growth Characteristics: Suspension growth
Culture System: IMDM + 20% FBS + 1% P/S
Matching Medium No.: TCH-G204
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma