This cell line is derived from the lymph nodes of a patient with undifferentiated gastric cancer and is capable of secreting mucin.
Cell Name: Human gastric carcinoma cell line (STR-authenticated)
Cell Synonyms: HGC-27; HGC 27; HGC27
Catalogue No.: TCH-C202
Species: Human
Tissue Origin: Stomach
Disease Feature: Gastric Cancer
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture System: RPMI-1640 + 10% FBS + 1% P/S
Matching Culture Medium No.: TCH-G202
Passage Ratio: 1:3–1:6, change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma