Cell Name: Human fetal lung fibroblast cell line
Cell Synonyms:HFL1; HFL-1; HFL 1; Human fetal lung fibroblast 1; HFL
Catalogue No.: TCH-C200
Species: Human
Tissue Origin: Lung
Disease Characteristics: Embryonic fibroblast
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: F12K + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G200
Passage Ratio: 1:3-1:4, with medium change every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions:: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended Starfish HyCyte® One-Step Cryopreservation Solution (Ready-to-use, serum-free, no programmed freezing required): No. GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma