Product Name：Luciferase Reporter Hep-G2 Cell Line

HyCyte cell line：HyCyte Hep-G2-Luc

Catalog No.：TCH-C196L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：Hep-G2

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Minimum Essential Medium (MEM) supplemented with 10 % FBS,4.0 ug/mL Puromycin,1%NEAA, 2mM Glutamax, 1mM Sodium Pyruvate,100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCH-G196L).

Passage：1:2-1:4

Doubling time：72-96 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：MEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).