Cell Name: Human hepatocellular carcinoma cell line (STR profile confirmed)
Cell Synonyms: Hep 3B2.1-7; Hep 3B2_1-7; HEP3B217; Hep 3B2; HEP-3B2; HEP3B2;   Hep-3B; HEP-3B; Hep 3B; Hep3B; HEP3B

Catalogue No.: TCH-C195

Species: Human

Tissue Origin: Liver
Disease Characteristic: Hepatocellular carcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% Glutamax+1% NEAA+1% Sodium Pyruvate+1% P/S

Matching Medium Catalog Number: TCH-G195

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 72-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection