Product Name：Luciferase Reporter HeLa Cell Line

HyCyte cell line：HyCyte HeLa-Luc

Catalog No.：TCH-C193L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：HeLa

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Minimum Essential Medium (MEM) supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/mlStreptomycin (HyCyte,TCH-G193L).

Passage：1:2-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.

Freezing media：MEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).