This cell line and its subline HEC-1-B were isolated in 1968 by H. Kuramoto and colleagues from a patient with stage IA endometrial cancer.

Cell Name: Human endometrial adenocarcinoma cell line

Cell Synonyms: HEC-1-A; Hec-1-A; HEC-1A; HEC1-A; HEC1A; Hec1A

Catalogue No.: TCH-C188

Species: Human

Tissue Origin: Uterus

Disease Characteristic: Endometrial Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: McCOY's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G332
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection