DNA fingerprinting evidence indicates that HCT-15 and DLD-1 cells are derived from the same individual; however, isoenzyme analysis and karyotyping still raise questions. HCT-15 cells are CSAp-negative (CSAp-); HCT-15 shows positive immunoperoxidase staining for keratin.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: HCT-15; HCT 15; HCT.15; HCT15

Catalogue No.:TCH-C186
Species: Human
Tissue Origin: Colorectum
Disease Characteristics: Colorectal adenocarcinoma

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G186

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection