Product Name：Luciferase Reporter HCT116 Cell Line

HyCyte cell line：HyCyte HCT116-Luc

Catalog No.：TCH-C185L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：HCT116

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium： McCoy's 5A Medium supplemented with 10 % FBS, 1.0ug/mL Puromycin,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G185L).

Passage：1:3-1:8

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:8.

Freezing media：McCoy's 5A+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).