HCT116 is one of three malignant cell lines isolated in 1979 by Brattain M et al. from a male patient with colon cancer; it produces CEA (carcinoembryonic antigen) and keratin. It forms colonies in semi-solid agarose culture medium. HCT116 is tumorigenic in athymic nude mice, forming epithelial-like tumors.

Cell Name: Human colorectal carcinoma cell line (STR-authenticated)

Cell Synonyms: HCT116; HCT-116; HCT.116; HCT_116; HCT116; CoCL2

Catalogue No.: TCH-C185

Species: Human
Tissue Origin: Colon
Disease Characteristic: Colon Cancer
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture Medium: McCoy's 5A + 10% FBS + 1% P/S
Matching Medium Code: TCH-G185
Passage Ratio: 1:3 to 1:8, change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma