This cell line was originally derived from a primary ductal carcinoma on October 13, 1995, and took 11.5 months to establish. The tumor was classified as TNM stage IIB, grade 3. BRCA1 analysis showed that this cell line is homozygous for the BRCA1 5382C mutation, whereas the lymphoblastoid cell line derived from the same patient is heterozygous at this mutation site. Two other family members also carry this mutation; one monozygotic twin sister also developed breast cancer. This cell line has an acquired TP53 mutation with loss of the wild-type allele, a homozygous deletion of the PTEN gene, and multiple heterozygous mutations at loci associated with breast cancer pathogenesis. This cell line is negative for both Her2-neu and p53 expression. HCC1937 is positive for the epithelial-specific markers epithelial glycoprotein 2 (EGP2) and cytokeratin 19. Estrogen receptor (ER) and progesterone receptor (PR) expressions are negative.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: HCC1937; HCC-1937; HCC/1937; Hamon Cancer Center 1937

Catalogue No.: TCH-C181

Species: Human
Tissue Origin: Breast
Disease Characteristics: Primary ductal carcinoma; Level 3, Stage IIB

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G181
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection