HBL-100 cells are an epithelial cell line established by E.V. Gaffney and colleagues from the breast milk of a donor with no family history of breast cancer. The karyotype of cultured HBL-100 cells became abnormal by the 7th passage. Electron microscopy images revealed the presence of microfilaments, tonofibrils, and desmosomes in HBL-100 cells. Southern blot analysis indicated that HBL-100 cells carry integrated SV40 viral genes and should not be considered normal cells.

Cell Name: Human breast epithelial cell line

Cell Synonyms: HBL-100; HBL 100; HBL100

Catalogue No.: TCH-C179
Species: Human
Tissue Origin: Mammary gland
Disease Characteristics: /
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G179
Passage Ratio: 1:2–1:4, with medium change every 2–3 days
Doubling Time: /
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma