The FaDu cell line was established in 1968 from a drill biopsy of a hypopharyngeal squamous carcinoma in an Indian patient. In FaDu cells, bundled filaments are observed in the cytoplasm, and the desmosomes at the cell boundaries are particularly prominent.

Cell Name: Human hypopharyngeal squamous cell carcinoma cell line

Cell Synonyms: FaDu; FaDU; FADU

Catalogue No.: TCH-C174

Species: Human

Tissue Origin: Hypopharynx

Disease Characteristics: Hypopharyngeal Squamous Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G174

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection