$ 800.00
Human umbilical vein endothelial cell hybrid line(EA.hy 926)
Cat.No. Size
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Cat.No.
TCH-C171
Size
1x10^6
Price
$800.00

The EA.hy926 cell line is a human umbilical vein endothelial cell line constructed by fusing primary cultured human umbilical vein cells with a thioguanine-resistant A549 clone under PEG (polyethylene glycol) induction. The hybrid cells were selected and grown in HAT medium, and further screened based on the presence of factor VIII-related antigen. The EA.hy926 cell line has undergone more than 100 population doublings (PDLs). Electron microscopy images show cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, demonstrating differentiated endothelial cell characteristics such as angiogenesis, homeostasis-thrombosis, blood pressure regulation, and inflammatory response.


Cell Name: Human umbilical vein endothelial cell hybrid line

Cell Synonyms: EA.hy926; EA. hy 926; EA hy 926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926

Catalogue No.: TCH-C171
Species: Human
Tissue Origin: Umbilical Vein

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G171

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


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