DU145 was established from a brain metastasis of a prostate cancer patient with a 3-year history of lymphocytic leukemia. This cell line shows no hormone sensitivity, is positive for acid phosphatase, and individual cells can form colonies in soft agar. Submicroscopic structural analysis of this cell line and the original tumor revealed microvilli, microfilaments, desmosomes, mitochondria, a well-developed Golgi apparatus, and heterogeneous lysosomes. The cells do not express prostate-specific antigen.

Cell Name: Human prostate carcinoma cell line (STR-authenticated)

Cell Synonyms: DU145; DU-145; Du-145; DU 145; DU_145; DU.145;   Duke University 145

Catalogue No.: TCH-C170

Species: Human
Tissue Source: Prostate
Disease Characteristic: Prostate Cancer
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture System: DMEM-H + 10% FBS + 1% P/S
Matching Medium No.: TCH-G170
Passage Ratio: 1:3–1:6, change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma