DLD-1 is one of two colorectal adenocarcinoma cell lines isolated by DL Dexter and colleagues between 1977 and 1979. It was found to have been contaminated with Mycoplasma hyorhinis when submitted to ATCC in 1979.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: DLD-1;DLD 1; DLD1; CoCL3

Catalogue No.: TCH-C169

Species: Human

Tissue Origin: Colorectum

Disease Characteristics: Colorectal adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G169

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection