The Daudi cell line was established in May 1967 by E. Klein and G. Klein from a 16-year-old Black male patient with Burkitt's lymphoma. The Daudi cells are surface immunoglobulin positive (sIg+), β2-microglobulin negative, Epstein-Barr nuclear antigen (EBNA) positive, and express the viral capsid antigen VCA; they carry the Epstein-Barr virus (EBV). Daudi cells are typical B lymphoblasts and are widely used in research on the pathogenesis of leukemia.

Cell Name: Human Burkitt's lymphoma cell line (STR authenticated)

Cell Synonyms: Daudi; DAUDI; NK-10A; NK-10a; NK 10a; NK10a; GM03190; GM3190; GM17346

Catalogue No.: TCH-C167

Species: Human

Tissue Origin: Peripheral blood

Disease Characteristics: Lymphoma

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G167

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection