This cell line was established in 1985 by Jacobsen PF, derived from a biopsy of a posterior fossa tumor in a 4-year-old child. No evidence of neuronal or glial differentiation has been detected; it can be used as a transfection host.

Cell Name: Human medulloblastoma cell line

Cell Synonyms: Daoy; DAOY; D324 Med; D-324 Med; D324 MED; D-324MED; D324

Catalogue No.: TCH-C166

Species: Human

Tissue Origin: Medulla

Disease Characteristic: Medulloblastoma

Morphology: Polygonal

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G166

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection