The CW-2 cell line is derived from human colon cancer and is CEA-positive. CW-2 cells can form tumors when transplanted into nude mice.

Cell Name: Human colorectal adenocarcinoma cell line

Cell Synonyms: CW-2; CW2

Catalogue No.: TCH-C164
Species: Human
Tissue Origin: Colon

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H+ 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G164

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection