Product Name：Luciferase Reporter COLO 205 Cell Line

HyCyte cell line：HyCyte COLO 205-Luc

Catalog No.：TCH-C161L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：COLO 205

Species：Human

Morphology：Epithelioid

Growth properties：Attached or floating

Culture Medium：RPMI-1640 supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G161L).

Passage：5×10^5-1×10^6cells/mL

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).