CFPAC-1 is a pancreatic ductal adenocarcinoma cell line derived from the liver metastasis of a 26-year-old Caucasian male with cystic fibrosis (CF). These cells express the cystic fibrosis transmembrane conductance regulator (CFTR) and exhibit an epithelial-like morphology with polarized apical microvilli. They also express pancreatic duct cell markers, including cytokeratins and carcinoembryonic antigen (CEA).CFPAC-1 is a subtriploid cell line, with 36% of cells having a modal chromosome number of 75. When passaged to the 24th generation, it can form tumors in immunosuppressed mice.

Cell Name: Human pancreatic ductal adenocarcinoma cell line

Cell Synonyms: CFPAC-1; CFPac-1; CF PAC-1; CF-PAC1; CF-Pac1; CF Pac1; CFPAC1; CFPac1; CFPAC

Catalogue No.: TCH-C157
Species: Human
Tissue Origin: Pancreas
Disease Characteristics: Pancreatic Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: IMDM + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G157

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection