Product Name：Luciferase Reporter CCRF-CEM Cell Line

HyCyte cell line：HyCyte CCRF-CEM-Luc

Catalog No.：TCH-C155L

Construction method：PiggyBac

Selection Marker：Luciferase

Parental：CCRF-CEM

Species：Human

Morphology：Lymphoblastic

Growth properties：Suspension

Culture Medium: RPMI-1640 supplemented with 10 % FBS,0.5 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G155L).

Passage: 3×10^5-5×10^5cells/mL

Doubling time: 24-48 h

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3

Freezing media: RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).