Calu-3 was established from the pleural effusion of a 25-year-old Caucasian male patient with lung adenocarcinoma; this patient had previously been treated with cyclophosphamide, bleomycin, and doxorubicin. Calu-3 cells are capable of forming tumors when inoculated into nude mice and can also serve as a host for transfection.

Cell Name: Human lung adenocarcinoma cell line

Cell Synonyms: CALU-3; CaLu-3; CALU-3; Calu 3; Calu3; CALU3

Catalogue No.: TCH-C151
Species: Human
Tissue Origin: Lung
Disease Characteristic: Lung Adenocarcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G151
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection