The ultrastructural features of Calu-1 cells include numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions, and the absence of viral particles. In addition, Calu-1 cells harbor the ras (H-ras) oncogene.

Cell Name: Human lung squamous cell carcinoma cell line

Cell Synonyms: CALU-1; CaLu-1; CALU 1; Calu 1; CALU1; Calu1

Catalogue No.: TCH-C150
Species: Human
Tissue Origin: Lung
Disease Characteristic: Lung Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: McCoy's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G150
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection