Human thyroid anaplastic carcinoma cells (Thyroid Anaplastic Carcinoma, ATC), undifferentiated, were established in 1988 from the thyroid (right lobe) of a 70-year-old woman with thyroid anaplastic carcinoma.

Cell Name: Human thyroid anaplastic carcinoma cell line

Cell Synonyms: CAL-62; Cal-62; CAL 62; Cal 62; CAL62; Centre Antoine Lacassagne-62

Catalogue No.: TCH-C149
Species: Human
Tissue Origin: Thyroid
Disease Characteristics: Thyroid Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H +10% FBS+1%Glutamax+1% Sodium Pyruvate+ 1% P/S
Matching Medium Catalog Number: TCH-G149
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 36-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection