This cell line was established in 1982 by J. Gioanni, derived from a lesion in the tongue of a 56-year-old Caucasian male, and shows strong positivity for keratin.

Cell Name: Human tongue squamous cell carcinoma cell line (STR authenticated)

Cell Synonyms: Cal-27; Cal-27; CAL 27; Cal 27; CAL27; Cal27;   Centre Antoine Lacassagne-27

Catalogue No.: TCH-C148

Species: Human
Tissue Origin: Tongue
Disease Characteristic: Squamous Cell Carcinoma
Morphology: Epithelial-like
Growth Properties: Adherent growth
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium No.: TCH-G148
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma