Caki-1's ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles.

Cell Name: Human renal clear cell carcinoma cell line
Cell Synonyms: CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1
Catalogue No.: TCH-C147
Species: Human
Tissue Origin: Kidney
Disease Characteristics: Clear cell renal cell carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: McCoy's 5A + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G147
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection