Product Name：Luciferase Reporter Caco-2 Cell Line

HyCyte cell line：HyCyte Caco-2-Luc

Catalog No.：TCH-C146L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：Caco-2

Species：Human

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose(DMEM)      supplemented with 10 % FBS,2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G146L）

Passage：1:3-1:4

Doubling time：60-80 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media：EMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).